Journal
NEUROPSYCHOPHARMACOLOGY
Volume 36, Issue 10, Pages 2103-2110Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/npp.2011.102
Keywords
basket cell; calcium/calmodulin-dependent kinase II alpha; dorsolateral prefrontal cortex; glutamic acid decarboxylase 65 kDa; parvalbumin; working memory
Categories
Funding
- NIH [F32 MH088160, MH084053, MH043784]
- BMS Foundation
- Bristol-Myers Squibb
- Curridium Ltd
- Pfizer
Ask authors/readers for more resources
Levels of messenger RNA (mRNA) for the alpha 1 subunit of the GABA(A) receptor, which is present in 60% of cortical GABA(A) receptors, have been reported to be lower in layer 3 of the prefrontal cortex (PFC) in subjects with schizophrenia. This subunit is expressed in both pyramidal cells and interneurons, and thus lower alpha 1 subunit levels in each cell population would have opposite effects on net cortical excitation. We used dual-label in situ hybridization to quantify GABA(A) alpha 1 subunit mRNA expression in calcium/calmodulin-dependent kinase II alpha (CaMKII alpha)-containing pyramidal cells and glutamic acid decarboxylase 65 kDa (GAD65)-containing interneurons in layer 3 of the PFC from matched schizophrenia and healthy comparison subjects. In subjects with schizophrenia, mean GABA(A) alpha 1 subunit mRNA expression was significantly 40% lower in pyramidal cells, but was not altered in interneurons. Lower alpha 1 subunit mRNA expression in pyramidal cells was not attributable to potential confounding factors, and thus appeared to reflect the disease process of schizophrenia. These results suggest that pyramidal cell inhibition is reduced in schizophrenia, whereas inhibition of GABA neurons is maintained. The cell type specificity of these findings may reflect a compensatory response to enhance layer 3 pyramidal cell activity in the face of the diminished excitatory drive associated with the lower dendritic spine density on these neurons. Neuropsychopharmacology (2011) 36, 2103-2110; doi: 10.1038/npp.2011.102; published online 15 June 2011
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available