Journal
NEUROPHARMACOLOGY
Volume 59, Issue 7-8, Pages 595-604Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2010.08.013
Keywords
Tanshinone IIA; beta-Amyloid; Cultured cortical neurons; Oxidative stress
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Oxidative stress caused by amyloid beta-peptide (A beta) may play an important role in the pathogenesis of Alzheimer disease (AD). All is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. Tanshinone IIA (Tan IIA) is extracted from a traditional herbal medicine Salvia miltiorrhiza BUNGE, which has been shown to protect against oxidative stress and cell death. In this study, we investigated the neuroprotective effect of Tan IIA against A beta(25-35)-induced cell death in cultured cortical neurons. Exposure of cortical neurons to 30 mu M A beta(25-35) caused a significant viability loss, cell apoptosis and decreased activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as increased levels of malondialdehyde (MDA) production. In parallel, A beta(25-35) significant increased the intracellular ROS elevation and decreased mitochondrial membrane potential (MMP). However, pretreatment of the cells with Tan IIA prior to A beta(25-35) exposure suppressed these A beta(25-35)-induced cellular events noticeably. In addition, Tan IIA reduced the A beta(25-35)-induced increase of caspase-3 activity, and reduced cytochrome c translocation into the cytosol from mitochondria. Furthermore, Tan IIA also ameliorated the A beta(25-35)-induced Bcl-2/Bax ratio reduction in cortical neurons. Taken together, these data indicate that Tan IIA protected cultured cortical neurons against A beta(25-35)-induced neurotoxicity through its antioxidative potential. Our results strongly suggest that Tan IIA may be effective in treating AD associated with oxidative stress. (C) 2010 Elsevier Ltd. All rights reserved.
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