Journal
NEUROPEPTIDES
Volume 43, Issue 2, Pages 63-71Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.npep.2009.01.004
Keywords
Goldfish; Orexin; NPY; Orexigenic action; Antagonists; Neuronal interaction; Double-immunostaining
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18370024]
- Toyama Marine Biotechnology Association
- Nissui Research Foundation
- Mishima Kalun Memorial Foundation
- Grants-in-Aid for Scientific Research [18370024] Funding Source: KAKEN
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Orexin-induced orexigenic action is mediated by neuropeptide Y (NPY) in goldfish and rodents. A previous study indicated that NPY-induced orexigenic action may also be mediated by orexin-A in goldfish. However, there is little information about the mutual actions of orexin-A and NPY in the goldfish. Therefore, using their specific receptor antagonists, we examined whether the orexigenic actions of orexin-A and NPY mutually interact in the goldfish. The stimulatory effect of intracerebroventricular injection of NPY at 1 pmol/g body weight (BW) on food intake was abolished by treatment with the orexin receptor-1 antagonist, SB334867, at 10 pmol/g BW whereas the NPY Y1-receptor antagonist, BIBP3226, at 100 pmol/g BW attenuated orexin-A (at 2.8 pmol/g BW)stimulated feeding. This led us, using a double-immunostaining method and confocal laser scanning microscopy, to investigate whether orexin-A- and NPY-containing neurons in the goldfish brain have direct mutual inputs. Orexin-A- and NPY-like immunoreactivities were distributed throughout the brain, especially in the diencephalon. Orexin-A- and NPY-containing neurons were located in a region of the hypothalamus, the nucleus posterioris periventricularis (NPPv), in close proximity to each other: NPY-containing nerve fibers or endings lay in close apposition to orexin-A-containing neurons in the NPPv, and orexin-A-containing nerve fibers or endings also lay in close apposition to NPY-containing neurons in the same region. These results indicate that, in goldfish, orexin-A- and NPY-induced orexigenic actions are mediated by mutual signaling pathways. (C) 2009 Elsevier Ltd. All rights reserved.
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