Journal
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
Volume 40, Issue 4, Pages 452-463Publisher
WILEY
DOI: 10.1111/nan.12078
Keywords
astrocyte; COX; microglia; neurone; neuropathic pain; spinal cord
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Funding
- Hong Kong Baptist University [FRG05-06/II-55, FRG/04-05/I-16]
- SCM postdoctoral fellowship
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AimsRecent studies suggested that the development of neuropathic pain associated with neural injury may be partly due to up-regulation of cyclooxygenase (COX) in the central nervous system. However, the cellular sources of COX-1 and COX-2 up-regulation following nerve injury are unclear. MethodsWe investigated the spinal cellular sources of COX-1 and COX-2 in association with allodynia following L5 spinal nerve ligation (SNL). ResultsPost-SNL pain-related behaviour was shown by increased sensitivity to mechanical stimulation. There was a significant increase in both COX-1 and COX-2 immunoreactivity (P<0.01) on the ipsilateral side of spinal dorsal horn. Double immunofluorescence labelling demonstrated that COX-1 immunoreactive cells colocalized chiefly with dorsal horn neuronal nuclei and microglia, whereas COX-2 was expressed in neuronal cytoplasm. ConclusionThese findings demonstrate that while spinal dorsal horn neurones are important source of COX-1 and COX-2 after nerve injury, microglia also contribute to the pathogenesis of neuropathic pain, partly by producing additional COX-1.
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