Journal
NEURON GLIA BIOLOGY
Volume 4, Issue -, Pages 179-187Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1740925X0999024X
Keywords
beta(3) integrin; synaptic homeostasis; synaptic scaling; mEPSCs; hippocampal organotypic slices
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Funding
- Medical Research Council
- MRC [MC_U122669937] Funding Source: UKRI
- Medical Research Council [MC_U122669937] Funding Source: researchfish
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Neuronal networks can adapt to global changes in activity levels through compensatory modifications in pre- and postsynaptic parameters of synaptic transmission. These forms of synaptic plasticity are known as synaptic homeostasis, and are thought to require specific cellular interactions and signaling across the entire neuronal network. However, the molecular mechanisms underlying synaptic homeostasis have so far been investigated mostly in primary cultures of dissociated neurons, a preparation that lacks the specificity of in vivo circuitry. Here, we show that there are critical differences in the properties of synaptic homeostasis between dissociated neuronal cultures and organotypic slices, a preparation that preserves more precisely in vivo connectivity. Moreover, the cell adhesion molecule beta(3) integrin, which regulates excitatory synaptic strength, is specifically required for a postsynaptic form of synaptic homeostasis called synaptic scaling in both dissociated cultures and organotypic slices. Conversely, another form of synaptic homeostasis that involves changes in presynaptic quantal content occurs independently of beta(3) integrin. Our findings define the differential involvement of beta(3) integrin in two forms of synaptic homeostasis.
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