Journal
NEURON
Volume 83, Issue 5, Pages 1058-1072Publisher
CELL PRESS
DOI: 10.1016/j.neuron.2014.07.024
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Funding
- Epilepsy Foundation Predoctoral Research Training Fellowship
- University of Utah Seed Grant Program
- NIH [K99/R00 NS076364, R21 OD016562, R03 MH103728, RC1 NS069033, R01 NS078331]
- Ben B. and Iris M. Margolis Foundation Award
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New strategies for introducing genetically encoded activity indicators into animal models facilitate the investigation of nervous system function. We have developed the PC:: G5-tdT mouse line that expresses the GCaMP5G calcium indicator in a Cre-dependent fashion. Instead of targeting the ROSA26 locus, we inserted the reporter cassette nearby the ubiquitously expressed Polr2a gene without disrupting locus integrity. The indicator was tagged with IREStdTomato to aid detection of positive cells. This reporter system is effective in a wide range of developmental and cellular contexts. We recorded spontaneous cortical calcium waves in intact awake newborns and evaluated concentration-dependent responses to odorants in the adult olfactory bulb. Moreover, PC:: G5-tdT effectively reports intracellular calcium dynamics in somas and fine processes of astrocytes and microglial cells. Through electrophysiological and behavioral analyses, we determined that GCaMP5G expression had no major impact on nervous system performance. PC:: G5-tdT will be instrumental for a variety of brain mapping experiments.
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