Journal
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
Volume 106, Issue -, Pages 20-27Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.sab.2015.01.011
Keywords
Boronic acid functionalized magnetic beads; Metal nanoparticle tag; Magnetic immunoassay; Glycoprotein quantification; Inductively coupled plasma mass spectrometry
Categories
Funding
- National Basic Research Program of China (973 Program) [2013CB933900]
- National Natural Science Foundation of China [21375097, 21205090, 21175102, 21075095]
- Science Fund for Creative Research Groups of NSFC [20621502, 20921062]
- SRFDP [20110141110010]
- Fundamental Research Funds for the Central Universities - Ministry of Education of China [114009]
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The absolute quantification of glycoproteins in complex biological samples is a challenge and of great significance. Herein, 4-mercaptophenylboronic acid functionalized magnetic beads were prepared to selectively capture glycoproteins, while antibody conjugated gold and silver nanoparticles were synthesized as element tags to label two different glycoproteins. Based on that, a new approach of magnetic immunoassay-inductively coupled plasma mass spectrometry (ICP-MS) was established for simultaneous quantitative analysis of glycoproteins. Taking biomarkers of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as two model glycoproteins, experimental parameters involved in the immunoassay procedure were carefully optimized and analytical performance of the proposed method was evaluated. The limits of detection (LODs) for AFP and CEA were 0.086 mu g L-1 and 0.054 mu g L-1 with the relative standard deviations (RSDs, n = 7, c = 5 mu g L-1) of 6.5% and 6.2% for AFP and CEA, respectively. Linear range for both AFP and CEA was 0.2-50 mu g L-1. To validate the applicability of the proposed method, human serum samples were analyzed, and the obtained results were in good agreement with that obtained by the clinical chemiluminescence immunoassay. The developed method exhibited good selectivity and sensitivity for the simultaneous determination of AFP and CEA, and extended the applicability of metal nanoparticle tags based on ICP-MS methodology in multiple glycoprotein quantifications. (C) 2015 Elsevier B.V. All tights reserved.
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