Journal
NEURON
Volume 68, Issue 1, Pages 99-112Publisher
CELL PRESS
DOI: 10.1016/j.neuron.2010.09.002
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Funding
- Japan Society for the Promotion of Science for Young Scientists
- NIH [5R01MH080379]
- Ministry of Education, Science, Sports and Culture of Japan
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Fasting has been used to control epilepsy since antiquity, but the mechanism of coupling between metabolic state and excitatory neurotransmission remains unknown. Previous work has shown that the vesicular glutamate transporters (VGLUTs) required for exocytotic release of glutamate undergo an unusual form of regulation by Cl-. Using functional reconstitution of the purified VGLUTs into proteoliposomes, we now show that Cl- acts as an allosteric activator, and the ketone bodies that increase with fasting inhibit glutamate release by competing with at the site of allosteric regulation. Consistent with these observations, acetoacetate reduced quantal size at hippocampal synapses and suppresses glutamate release and seizures evoked with 4-aminopyridine in the brain. The results indicate an unsuspected link between metabolic state and excitatory neurotransmission through anion-dependent regulation of VGLUT activity.
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