Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 150, Issue -, Pages 786-791Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2015.06.032
Keywords
Carmine; Triton X-100; Cloud point extraction; Spectrophotometric; Food samples
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Funding
- Islamic Azad University, Arak Branch
- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences
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In this paper, a simple and cost effective method was developed for extraction and pre-concentration of carmine in food samples by using cloud point extraction (CPE) prior to its spectrophotometric determination. Carmine was extracted from aqueous solution using Triton X-100 as extracting solvent. The effects of main parameters such as solution pH, surfactant and salt concentrations, incubation time and temperature were investigated and optimized. Calibration graph was linear in the range of 0.04-5.0 mu g mL(-1) of carmine in the initial solution with regression coefficient of 0.9995. The limit of detection (LOD) and limit of quantification were 0.012 and 0.04 mu g mL(-1), respectively. Relative standard deviation (RSD) at low concentration level (0.05 mu g mL(-1)) of carmine was 4.8% (n = 7). Recovery values in different concentration levels were in the range of 93.7-105.8%. The obtained results demonstrate the proposed method can be applied satisfactory to determine the carmine in food samples. C) 2015 Elsevier B.V. All rights reserved.
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