Journal
NEURON
Volume 59, Issue 6, Pages 1024-1036Publisher
CELL PRESS
DOI: 10.1016/j.neuron.2008.07.036
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Funding
- Howard Hughes Medical Institute
- National Institutes of Health [MH37134]
- Advanced Studies in America
- Brain Research Center Frontier Program [M103KV010008-06K2201-00810]
- Korea Ministry of Science and Technology
- Kavli Institute for Brain Sciences
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The time course of the requirement for local protein synthesis in the stabilization of learning-related synaptic growth and the persistence of long-term memory was examined using Aplysia bifurcated sensory neuron-motor neuron cultures. We find that, following repeated pulses of serotonin (5-HT), the local perfusion of emetine, an inhibitor of protein synthesis, or a TAT-AS oligonucleotide directed against ApCPEB blocks long-term facilitation (LTF) at either 24 or 48 hr and leads to a selective retraction of newly formed sensory neuron varicosities induced by 5-HT. By contrast, later inhibition of local protein synthesis, at 72 hr after 5-HT, has no effect on either synaptic growth or LTF. These results define a specific stabilization phase for the storage of long-term memory during which newly formed varicosities are labile and require sustained CPEB-dependent local protein synthesis to acquire the more stable properties of mature varicosities required for the persistence of LTF.
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