Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 149, Issue -, Pages 674-681Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2015.04.111
Keywords
Chemosensor; Ferric ion; Detection; Fluorescence imaging; Flow cytometry
Categories
Funding
- National Natural Science Foundation of China [21301011]
- State Key Laboratory of Fine Chemicals [KF1305]
- Macquarie University Research Fellowship Scheme [MQRF-1487520]
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Although ferric ion (Fe3+) performs critical roles in diverse biochemical processes in living systems, its physiological and pathophysiological functions have not been fully explored due to the lack of methods for quantification of Fe3+ ions in biological system. In this work, a highly sensitive and selective fluorescence chemosensor, L, was developed for the detection of Fe3+ ions in aqueous solution and in living cells. L was facile synthesized by one step reaction and well characterized by NMR, API-ES, FT-IR, and elementary analysis. The prepared chemosensor displayed excellent selectivity for Fe3+ ions detection over a wide range of tested metal ions. In the present of Fe3+ ions, the strong green fluorescence of L was substantially quenched. The 1:1 stoichiometry of the complexation was confirmed by a Job's plot. The association constant (K-a) of L with Fe3+ was evaluated using the Benesi-Hildebrand method and was found to be 1.36 x 10(4) M-1. The MIT assay determined that L exhibits low cytotoxicity toward living cells. Confocal imaging and flow cytometry studies showed that L is readily interiorized by MDA-MB-231 cells through an energy-dependent pathway and could be used to detect of Fe3+ ions in living cells. (C) 2015 Elsevier B.V. All rights reserved.
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