Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 138, Issue -, Pages 381-386Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2014.11.080
Keywords
Gold nanoparticles (AuNPs); Creatinine; Surface plasmon resonance (SPR); Human urine; Solid phase extraction
Categories
Funding
- Royal Golden Jubilee (RGJ) Ph.D. Program from the Thailand Research Fund [PHD/0266/2552]
- Environmental Analysis Research Unit (EARU)
- Department of Chemistry, Chulalongkorn University
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Label-free gold nanoparticles (AuNPs) were utilized in the detection of creatinine in human urine after a sample preparation by extraction of creatinine on sulfonic acid functionalized silica gel. With the proposed sample preparation method, the interfering effects of the urine matrix on creatinine detection by AuNPs were eliminated. Parameters affecting creatinine extraction were investigated. The aggregation of AuNPs induced by creatinine resulted in a change in the surface plasmon resonance signal with a concomitant color change that could be observed by the naked eye and quantified spectrometrically. The effect of AuNP concentration and reaction time on AuNP aggregation was investigated. The method described herein provides a determination of creatinine in a range of 15-40 mg L-1 with a detection limit of 13.7 mg L-1 and it was successfully used in the detection of creatinine in human urine samples. (C) 2014 Elsevier B.V. All rights reserved.
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