Journal
NEUROIMMUNOMODULATION
Volume 19, Issue 2, Pages 69-78Publisher
KARGER
DOI: 10.1159/000329002
Keywords
Stress; Spleen; T cells; B cells; beta-Adrenergic receptors; Apoptosis
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Funding
- VEGA [2/0036/11, 2/0188/09, 2/0049/10]
- NFM/EEA [SK0095]
- Nadacia Intenda
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Objectives: Stress-induced rise in circulating catecholamines (CAs), followed by modulation of beta-adrenergic receptors (adrenoceptors, ARs), is one of the pathways involved in the stress-mediated effects of immune functions. The spleen is an organ with a high number of lymphocytes and provides a unique microenvironment in which they reside. Thus, lymphocytes may respond differently to CAs in the spleen than in the circulation. No reports exist concerning the involvement of beta-ARs in stress-mediated effects on T and B cells isolated from the spleen. Therefore, our aim was to investigate the effect of single stress exposure on gene expression and cellular localization of alpha-adrenoceptor subtypes in splenic T and B cells. We tried to correlate changes in adrenoceptors with the expression of apoptotic proteins. Methods: Immobilization (IMMO) was used as a stress model. T and B cells were isolated from rat spleen using magnetically labeled antibodies. The gene expression of individual adrenoceptors and apoptotic proteins was evaluated by real-time PCR. Immunofluorescence was used to evaluate localization and adrenoceptor expression. Results: We have found T cells to be more vulnerable to stress compared to B cells, because of increased beta(1)-, beta(2)- and beta(3)-ARs after a single IMMO. Moreover, beta(2)-ARs translocated from the nucleus to the plasma membrane in T cells after IMMO. The rise in beta-ARs most probably led to the rise of Bax mRNA and Bax to Bcl-2 mRNA ratio. This might suggest the induction of an apoptotic process in T cells. Conclusion: Higher susceptibility of T cells to stress via modulation of beta-ARs and apoptotic proteins might shift the immune responsiveness in the spleen. Copyright (C) 2012 S. Karger AG, Basel
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