Journal
NEUROCHEMISTRY INTERNATIONAL
Volume 55, Issue 4, Pages 208-213Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2009.02.024
Keywords
Treadmill exercise; Hippocampus; Induced aging rats; NGF; PI3-K/Akt; Apoptosis
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Funding
- Korean Government (MOEHRD) [KRF-2007313-G00006]
- National Research Foundation of Korea [2007-313-G00006] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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While nerve growth factor (NGF) activates various signaling cascades, the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway plays a pivotal role in controlling the survival of neurons, although this activity declines during the aging process. We investigated the effect of forced moderate-intensity treadmill exercise on the level of NGF and the PI3-K/Akt signaling pathway in the hippocampus of induced aging rats. Forty-five male Sprague-Dawley rats were divided into the following three groups: (1) control group, in which aging was not induced (CON: n=15), (2) aging-control group, in which aging was induced but the rats were not subjected to exercise (ACON: n = 15), and (3) the aging-exercise group, in which aging was induced and the rats were subjected to treadmill exercise (AEX: n = 15). D-Galactose (50 mg/kg) was injected into the abdominal cavity for 8 weeks to induce aging. Rats were subjected to treadmill exercise 5 days a week for 8 weeks, and the speed of the treadmill was gradually increased. The protein levels of NGF, P-PI3-K, and P-Akt were significantly high in the AEX group (p < 0.01, p < 0.01, and p < 0.001, respectively). Tyrosine kinase A (Trk A) receptor level was significantly higher in the CON and AEX groups than in the ACON group (p < 0.01). TUNEL assay showed a significant reduction in apoptosis in the AEX group (p < 0.001). Caspase-3 activation was significantly decreased in the AEX and CON groups (p < 0.05). These results show that forced moderate-intensity treadmill exercise increases the level of NGF and activates P-PI3-K to induce P-Akt in order to suppress apoptotic cell death in the hippocampus of induced aging rats. (C) 2009 Elsevier Ltd. All rights reserved.
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