Journal
NEUROCHEMISTRY INTERNATIONAL
Volume 55, Issue 7, Pages 710-716Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2009.07.002
Keywords
Cell senescence; SA-beta-gal; Simulated microgravity; Oxidant stress; PC12 cell
Categories
Funding
- Harbin Medical University Youth Science Found [060038]
- Harbin Medical University Innovation Found [HCXB2008004]
- Sanitary Bureau of Heilongjiang Province [2005-15]
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Microgravity has a unique effect on biological organisms. Organs exposed to microgravity display cellular senescence, a change that resembles the aging process. To directly investigate the influence of simulated microgravity on neuronal original rat PC12 cells, we used a rotary cell culture system that simulates the microgravity environment on the earth. We found that simulated microgravity induced partial G1 phase arrest, upregulated senescence-associated P-galactosidase (SA-p-gal) activity, and activated both p53 and p16 protein pathways linked to cell senescence. The amount of reactive oxygen species (ROS) was also increased. The activity of intracellular antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), was all significantly increased at 12 h after the microgravity onset, yet decreased at 96 h. Furthermore, concomitant block of ROS by the antioxidant N-acetylcysteine significantly inhibited the microgravity-induced upregulation of SA-beta-gal activity. These results suggest that exposure to simulated microgravity induces cellular senescence in PC12 cells via an increased oxidant stress. (C) 2009 Elsevier Ltd. All rights reserved.
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