Journal
NEUROBIOLOGY OF LEARNING AND MEMORY
Volume 95, Issue 4, Pages 498-504Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nlm.2011.02.013
Keywords
Arc/Arg3.1; catFISH; Fear conditioning; Imaging; Memory; Off-line processing
Funding
- Japan Science Society
- Takeda Science Foundation
- University of Tokyo/Leica microsystems imaging center
- [19390019]
- Grants-in-Aid for Scientific Research [22390012] Funding Source: KAKEN
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Information processing in the central nervous system (CNS) during periods of rest is crucial for lasting memories but the precise off-line neuronal population activity that contributes to long-term memory formation remains unclear. This pattern of neuronal activity during rest triggers transcription of immediate early genes such as activity regulated cytoskeletal gene (Arc). We compared the active neuronal population in the lateral amygdala of C57BL/6 J mice during fear conditioning and rest periods using a large scale imaging technique, Arc cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH). We found that the neuronal population transcribing Arc during fear conditioning was more similar to that the population transcribing Arc after fear conditioning than before fear conditioning. The overlapping population was larger in conditioned mice that acquired associative memory than in unshocked mice and in latent inhibited mice that received shocks but did not form associative memory. Moreover, these results were confirmed using Arc/Homer la catFISH. Our findings indicate that Arc is preferentially transcribed in neurons that are active during fear conditioning after associative learning. This preferential transcription may contribute to the formation of long-lasting memory. (C) 2011 Elsevier Inc. All rights reserved.
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