Journal
NEUROBIOLOGY OF AGING
Volume 35, Issue 8, Pages 1947-1955Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2014.01.136
Keywords
Two-photon microscopy; Cerebral blood flow; Aging; Capillary; Rat; Hematocrit
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Funding
- National Science and Engineering Research Council of Canada
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Age-related decreases in baseline cerebral blood flow have been measured with various imaging modalities, however, the contribution of capillary flow to this phenomenon remain to elucidate. This study used 2-photon laser scanning fluorescence microscopy to measure capillary diameter, red blood cell speed, and flux in individual capillaries in the sensory-motor cortex of 12 adult (3-month-old) and 12 old (24-month-old) male Long-Evans rats under isoflurane anesthesia. The average (+/- standard deviation) diameter and speed over 921 capillaries were 6.4 +/- 1.4 mu m and 1.3 +/- 1.1 mm/s, respectively. Red blood cell speed and flux were significantly higher, by 48% and 15%, respectively, in old compared with young animals (p < 5%). The diameter also showed a similar tendency (7% higher, p = 5.7%). Furthermore, capillary hematocrit and density were significantly lower in the older group (p < 5%), by 32% and 20%, respectively. (C) 2014 Elsevier Inc. All rights reserved.
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