Journal
NEUROBIOLOGY OF AGING
Volume 31, Issue 4, Pages 696-705Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2008.05.024
Keywords
Circadian; Age; Pituitary; Rhesus macaque
Categories
Funding
- NIH [AG-021380, AG-029612, HD-029186, RR-00163]
- Collins Medical Trust
- Department of Animal and Avian Sciences at the University of Maryland
- National Institute on Aging
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [R01HD029186] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [K01RR000163, P51RR000163] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG029612] Funding Source: NIH RePORTER
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Recent studies have shown that circadian clock genes are expressed in various peripheral tissues, raising the possibility that multiple clocks regulate circadian physiology To study clock gene expression in the rhesus macaque pituitary gland we used gene microarray data and found that the pituitary glands of young and old adult males express several components of the circadian clock (Per1, Per2, Cry1, Bolan1, Clock, Rev-erb alpha and Csnk1 epsilon) Semi-quantitative reverse-transcription polymerase chain reaction (sqRT-PCR) confirmed the presence of these core-clock genes and detected significant age-related differences in the expression of Per2 sqRT-PCR also showed differential expression of core-clock genes at two opposing time-points over the 24-h day, with greater expression of Per2 and Bmal1 (P <0 05) at 1300 h as compared to 0100 h Immunohistochemistry revealed rhythmic expression of REV-ERB alpha in the pituitary glands of female macaques These data provide evidence that the rhesus macaque pituitary gland expresses core-clock genes and their associated protein products in a 24-h rhythmic pattern, and that their expression is moderately impacted by aging processes Published by Elsevier Inc
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