Journal
NEURO-ONCOLOGY
Volume 14, Issue 8, Pages 1026-1036Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/nos122
Keywords
glioma; HIF-1; microRNA; VHL; -catenin
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Funding
- National High Technology Research and Development Program 863 [2012AA02A508]
- China National Natural Scientific Fund [30971136, 81001128, 81071044]
- Program for New Century Excellent Talents in University [NCET-07-0615]
- Tianjin Science and Technology Committee [09JCZDJC17600]
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Aberrant microRNA expression has been implicated in the development of human cancers. Here, we investigated the oncogenic significance and function of miR-23b in glioma. We identified that the expression of miR-23b was elevated in both glioma samples and glioma cells, indicated by real-time polymerase chain reaction analyses. Down-regulation of miR-23b triggered growth inhibition, induced apoptosis, and suppressed invasion of glioma in vitro. Luciferase assay and Western blot analysis revealed that VHL is a direct target of miR-23b. Restoring expression of VHL inhibited glioma proliferation and invasion. Mechanistic investigation revealed that miR-23b deletion decreased HIF-1/VEGF expression and suppressed -catenin/Tcf-4 transcription activity by targeting VHL. Furthermore, expression of VHL was inversely correlated with miR-23b in glioma samples and was predictive of patient survival in a retrospective analysis. Therefore, we demonstrated that downregulation of miR-23b suppressed tumor survival through targeting VHL, leading to the inhibition of -catenin/Tcf-4 and HIF-1/VEGF signaling pathways.
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