Journal
NEURO-ONCOLOGY
Volume 14, Issue 11, Pages 1332-1345Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/nos247
Keywords
B-crystallin; apoptosis; glioma; SAHA; XIAP
Categories
Funding
- National Research Foundation of Korea
- Korean government [2010-0001942]
- National Research Foundation of Korea [2009-0093193, 핵06B3503] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Although crystallins are major structural proteins in the lens, -crystallins perform non-lens functions, and B-crystallin has been shown to act as an anti-apoptotic mediator in various cells. The present study was undertaken to examine whether B-crystallin expressed in human malignant glioma cells exerts anti-apoptotic acitivity. In addition, we sought to elucidate the mechanism underlying any observed anti-apoptotic function of B-crystallin in these cells. Three glioma cell lines, U373MG, U118MG, and T98G, were used. We observed that only the U373MG cell line expresses B-crystallin, whereas the other 2 glioma cell lines, U118MG and T98G, demonstrated no endogenous expression of B-crystallin. We next observed that the silencing of B-crystallin sensitized U373MG cells to suberoylanilide hydroxamic acid (SAHA)induced apoptosis and that B-crystallin associates with caspase-3 and XIAP. Because XIAP is the most potent suppressor of mammalian apoptosis through the direct binding with caspases, we assessed whether XIAP also plays an anti-apoptotic role in SAHA-induced apoptosis in B-crystallin-expressing U373MG cells. Of note, the silencing of XIAP did not alter the amount of cell death induced by SAHA, indicating that XIAP does not exert an anti-apoptotic activity in U373MG cells. We then determined whether the ectopic expression of B-crystallin in glioma cells caused a loss of the anti-apoptotic activity of XIAP. Accordingly, we established 2 B-crystallin over-expressing glioma cell lines, U118MG and T98G, and found that the silencing of XIAP did not sensitize these cells to SAHA-induced apoptosis. These findings suggest that B-crystallin expressed in glioma cells overrides the anti-apoptotic activity exerted by XIAP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available