Journal
NEPHROLOGY DIALYSIS TRANSPLANTATION
Volume 24, Issue 10, Pages 3024-3032Publisher
OXFORD UNIV PRESS
DOI: 10.1093/ndt/gfp214
Keywords
IL-1; interstitial fibrosis; macrophages; obstructive uropathy; TGF-beta
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Funding
- NHMRC of Australia [334067]
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Background. IL-1 beta has the potential to promote progressive renal disease by effects on macrophage recruitment and activation or by effects mediated through tubular cell transforming growth factor (TGF)-beta production, previously demonstrated in vitro. Methods. The in vivo roles of endogenous IL-1 beta and its type I receptor (IL-1RI) in renal fibrosis were studied using wild-type C57BL/6 mice, IL-1 beta(-/-) and IL-1RI(-/-) mice with unilateral ureteric obstruction. Results. After 7 days, IL-1RI(-/-) mice (IL-1 alpha and IL-1 beta deficient) were protected from injury and collagen accumulation. IL-1 beta(-/-) mice demonstrated some histological protection, but no reduction in alpha 1(1) procollagen mRNA or biochemically measured collagen accumulation. Compared with obstructed kidneys from wild-type mice, TGF-beta 1 mRNA was reduced in IL-1RI(-/-) mice (with trends to reduced TGF-beta 2 and TGF-beta 3). Expression of a downstream TGF-beta effector, connective tissue growth factor, was decreased in IL-1RI(-/-) mice. IL-1RI(-/-) mice exhibited less tubulointerstitial apoptosis compared with wild-type mice. Macrophage infiltration and adhesion molecule mRNA expression was unchanged in IL-1 beta(-/-) or IL-1RI(-/-) mice. While TNF expression was similar to wild-type mice, IFN-gamma expression was reduced in both IL-1 beta(-/-) and IL-1RI(-/-) mice. IL-1RI(-/-) mice at 14 days showed a catch-up in fibrosis compared with wild-type mice. Conclusion. IL-1/IL-1RI interactions are profibrotic in renal fibrosis. IL-1RI(-/-) mice were more protected at an early stage, associated with changes in TGF-beta and downstream mediators of fibrosis, but independent of the presence of infiltrating macrophages.
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