Journal
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Volume 379, Issue 4, Pages 397-402Publisher
SPRINGER
DOI: 10.1007/s00210-009-0393-0
Keywords
Antibody selectivity; Antibody specificity; Immunohistochemistry; Western blotting
Categories
Funding
- John L. Emmett Foundation for Urology
- The Netherlands
- Thai government [008/2550, 125/2550]
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Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M1-4 muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
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