Journal
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Volume 379, Issue 4, Pages 409-412Publisher
SPRINGER
DOI: 10.1007/s00210-008-0368-6
Keywords
Alpha-1-adrenoceptor; Antibodies; Subtypes
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Funding
- United States Department of Veterans Affairs Research Service
- NIH
- GlaxoSmithKline Research Foundation
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Commercial antibodies are used widely to quantify and localize the alpha 1-adrenergic receptor (AR) subtypes, alpha 1A, alpha 1B, and alpha 1D. We tested ten antibodies, from abcam and Santa Cruz, using western blot with heart and brain tissue from wild-type (WT) mice and mice with systemic knockout (KO) of one or all three subtypes. We found that none of the antibodies detected a band in WT that was absent in the appropriate KO or in the KO that was null for all alpha 1-ARs (ABDKO). We conclude that the antibodies we tested are not specific for alpha 1-ARs. These results raise caution with prior studies using these reagents. For now, competition radioligand binding is the only reliable approach to quantify the alpha 1-AR subtype proteins. Receptor protein localization remains a challenge.
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