Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 21, Issue 2, Pages 126-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2746
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Funding
- G. Harold & Leila Y. Mathers Charitable Foundation
- US National Institutes of Health Training [T32NS007484]
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The mammalian circadian clock is built on a molecular feedback loop in which the Period (PER) proteins, acting in a large, poorly understood complex, repress Clock-Bmal1, the transcription factor driving their expression. We found that mouse PER complexes include the histone methyltransferase HP1 gamma-Suv39h. PER proteins recruited HP1 gamma-Suv39h to the Per1 and Per2 promoters, and HP1 gamma-Suv39h proved important for circadian di- and trimethylation of histone H3 Lys9 (H3K9) at the Per1 promoter, feedback repression and clock function. HP1 gamma-Suv39h was recruited to the Per1 and Per2 promoters similar to 4 h after recruitment of HDAC1, a PER-associated protein previously implicated in clock function and H3K9 deacetylation at the Per1 promoter. PER complexes containing HDAC1 or HP1 gamma-Suv39h appeared to be physically separable. Circadian clock negative feedback by the PER complex thus involves dynamic, ordered recruitment of repressive chromatin modifiers to DNA-bound Clock-Bmal1.
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