Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 20, Issue 11, Pages 1321-U252Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2680
Keywords
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Funding
- European Molecular Biology Organization (EMBO) [ALTF864-2008, ALTF864-2009]
- Japan Society for the Promotion of Science postdoctoral fellowship
- Agence Nationale de la Recherche [ANR-09-Blanc-0114]
- EpiGeneSys Network of Excellence, EMBO Yong Investigator Programme
- European Research Council Starting Grant (ERC-Stg 'NuclearPotency')
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The spatiotemporal organization of genomes in the nucleus is an emerging key player to regulate genome function. Live imaging of nuclear organization dynamics would be a breakthrough toward uncovering the functional relevance and mechanisms regulating genome architecture. Here, we used transcription activator-like effector (TALE) technology to visualize endogenous repetitive genomic sequences. We established TALE-mediated genome visualization (TGV) to label genomic sequences and follow nuclear positioning and chromatin dynamics in cultured mouse cells and in the living organism. TGV is highly specific, thus allowing differential labeling of parental chromosomes by distinguishing between single-nucleotide polymorphisms (SNPs). Our findings provide a framework to address the function of genome architecture through visualization of nuclear dynamics in vivo.
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