Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 18, Issue 10, Pages 1124-U58Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2116
Keywords
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Funding
- Swiss National Science Foundation [GM56888, PBZH33-112756, PA0033-117484]
- Eugen and Elisabeth Schellenberg Foundation [BFU2006-05260]
- Spanish Ministry of Science and Innovation [Consolider Ingenio 2010 CSD2007-015]
- [ES07061]
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The Mre11 complex (Mre11, Rad50 and Xrs2 in Saccharomyces cerevisiae) influences diverse functions in the DNA damage response. The complex comprises the globular DNA-binding domain and the Rad50 hook domain, which are linked by a long and extended Rad50 coiled-coil domain. In this study, we constructed rad50 alleles encoding truncations of the coiled-coil domain to determine which Mre11 complex functions required the full length of the coils. These mutations abolished telomere maintenance and meiotic double-strand break (DSB) formation, and severely impaired homologous recombination, indicating a requirement for long-range action. Nonhomologous end joining, which is probably mediated by the globular domain of the Mre11 complex, was also severely impaired by alteration of the coiled-coil and hook domains, providing the first evidence of their influence on this process. These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50.
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