Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 7, Pages 909-U166Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1838
Keywords
-
Funding
- European Research Council [206726-CLIP]
- Human Frontiers Science Program [RGP0024]
- BBSRC [BB/E01075X/1] Funding Source: UKRI
- MRC [MC_U105185858] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E01075X/1] Funding Source: researchfish
- Medical Research Council [MC_U105185858] Funding Source: researchfish
Ask authors/readers for more resources
In the nucleus of eukaryotic cells, nascent transcripts are associated with heterogeneous nuclear ribonucleoprotein (hnRNP) particles that are nucleated by hnRNP C. Despite their abundance, however, it remained unclear whether these particles control pre-mRNA processing. Here, we developed individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to study the role of hnRNP C in splicing regulation. iCLIP data show that hnRNP C recognizes uridine tracts with a defined long-range spacing consistent with hnRNP particle organization. hnRNP particles assemble on both introns and exons but remain generally excluded from splice sites. Integration of transcriptome-wide iCLIP data and alternative splicing profiles into an 'RNA map' indicates how the positioning of hnRNP particles determines their effect on the inclusion of alternative exons. The ability of high-resolution iCLIP data to provide insights into the mechanism of this regulation holds promise for studies of other higher-order ribonucleoprotein complexes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available