Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 10, Pages 1210-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1901
Keywords
-
Funding
- US National Institutes of Health [GM045948]
Ask authors/readers for more resources
E. coli RecBCD is a DNA helicase with two ATPase motors (RecB, a 3'-> 5' translocase, and RecD, a 5'-> 3' translocase) that function in repair of double-stranded DNA breaks. The RecBC heterodimer, with only the RecB motor, remains a processive helicase. Here we examined RecBC translocation along single-stranded DNA (ssDNA). Notably, we found RecBC to have two translocase activities: the primary translocase moves 3'-> 5', whereas the secondary translocase moves RecBC along the opposite strand of a forked DNA at a similar rate. The secondary translocase is insensitive to the ssDNA backbone polarity, and we propose that it may fuel RecBCD translocation along double-stranded DNA ahead of the unwinding fork and ensure that the unwound single strands move through RecBCD at the same rate after interaction with a crossover hot-spot indicator (Chi) sequence.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available