Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 10, Pages 1263-1265Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1905
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Funding
- Cancer Research UK
- Breast Cancer Campaign
- Louis-Jeantet Foundation
- European Research Council, Swiss Bridge
- US National Institutes of Health
- Alfred Benzon Foundation
- Carlsberg Foundation
- European Molecular Biology Organization
- Cancer Research UK [11582] Funding Source: researchfish
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Individuals with BRCA2 mutations are predisposed to breast cancers owing to genome instability. To determine the functions of BRCA2, the human protein was purified. It was found to bind selectively to single-stranded DNA (ssDNA), and to ssDNA in tailed duplexes and replication fork structures. Monomeric and dimeric forms of BRCA2 were observed by EM. BRCA2 directed the binding of RAD51 recombinase to ssDNA, reduced the binding of RAD51 to duplex DNA and stimulated RAD51-mediated DNA strand exchange. These observations provide a molecular basis for the role of BRCA2 in the maintenance of genome stability.
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