Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 4, Pages 430-U68Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1780
Keywords
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Funding
- US National Institutes of Health [R01 GM078259]
- Cancer Center [P30 CA15704]
- NATIONAL CANCER INSTITUTE [P30CA015704] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM078259] Funding Source: NIH RePORTER
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Although histone modifications have been implicated in many DNA-dependent processes, their precise role in DNA replication remains largely unknown. Here we describe an efficient single-step method to specifically purify histones located around an origin of replication from Saccharomyces cerevisiae. Using high-resolution MS, we have obtained a comprehensive view of the histone modifications surrounding the origin of replication throughout the cell cycle. We have discovered that acetylation of histone H3 and H4 is dynamically regulated around an origin of replication, at the level of multiply acetylated histones. Furthermore, we find that this acetylation is required for efficient origin activation during S phase.
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