Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 5, Pages 590-U88Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1820
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Funding
- UK Medical Research Council
- Royal Society UK
- US National Institutes of Health
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Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification of their mechanochemical cycles. Here we used an optical trap-based assay to investigate the reversibility of the force-generating conformational change ( power stroke) of single myosin-Va motor heads. By applying load to the head shortly after binding to actin, we found that, at a certain load, the power stroke could be reversed, and the head fluctuated between an actin-bound pre- and a post-power stroke conformation. This load-dependent mechanical instability might be critical to coordinate the heads of processive, dimeric myosin-Va. Nonlinear response to load leading to coordination or oscillations amongst motors might be relevant for many cellular functions.
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