Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 17, Issue 6, Pages 775-U152Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1811
Keywords
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Funding
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinschaft [SFB 638/B2]
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Positional knowledge of subunits within multiprotein assemblies is crucial for understanding their function. The topological analysis of protein complexes by electron microscopy has undergone impressive development, but analysis of the exact positioning of single subunits has lagged behind. Here we have developed a clonable similar to 80-residue tag that, upon attachment to a target protein, can recruit a structurally prominent electron microscopy label in vitro. This tag is readily visible on single particles and becomes exceptionally distinct after image processing and classification. Thus, our method is applicable for the exact topological mapping of subunits in macromolecular complexes.
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