Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 16, Issue 11, Pages 1167-U7Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1686
Keywords
-
Funding
- MDACC, ACS [RSG-05-060-01-GMC]
- National Institute of Environmental Health Sciences [ES07784]
- Rosalie B.Hite Fellowship
- Fondation Recherche Medicale
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche and the Institut National du Cancer
- ARC
- US National Institutes of Health [GM71729]
- Ministry of Education Science and Sports (MEXT), Japan
Ask authors/readers for more resources
ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling complex in the DNA damage tolerance pathways. Whereas INO80 is necessary for the resumption of replication at forks stalled by methyl methane sulfonate (MMS), it is not required for replication fork collapse after treatment with hydroxyurea (HU). Mechanistically, INO80 regulates DNA damage tolerance during replication through modulation of PCNA (proliferating cell nuclear antigen) ubiquitination and Rad51-mediated processing of recombination intermediates at impeded replication forks. Our findings establish a mechanistic link between INO80 and DNA damage tolerance pathways, indicating that chromatin remodeling is important for accurate DNA replication.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available