Journal
NATURE REVIEWS MOLECULAR CELL BIOLOGY
Volume 13, Issue 11, Pages 736-742Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nrm3453
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Funding
- Swiss National Science Foundation (SNSF)
- National Center for Competence in Research in Structural Biology
- Swiss National Science Foundation [SNSF 31003A_141083/1]
- European Research Council (ERC) [243047 INCEL]
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Visualizing the dynamic molecular architecture of cells is instrumental for answering fundamental questions in cellular and structural biology. Although modern microscopy techniques, including fluorescence and conventional electron microscopy, have allowed us to gain insights into the molecular organization of cells, they are limited in their ability to visualize multicomponent complexes in their native environment. Cryo-electron tomography (cryo-ET) allows cells, and the macromolecular assemblies contained within, to be reconstructed in situ, at a resolution of 2-6 nm. By combining cryo-ET with super-resolution fluorescence microscopy approaches, it should be possible to localize proteins with high precision inside cells and so elucidate a more realistic view of cellular processes. Thus, cryo-ET may bridge the resolution gap between cellular and structural biology.
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