Journal
NATURE REVIEWS GENETICS
Volume 15, Issue 5, Pages 321-334Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nrg3686
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Funding
- National Research Foundation of Korea [2013-000718, 2011-0013568]
- Health Technology Research and Development Project by the Korean Ministry of Health and Welfare [H10C1740]
- Converging Research Center Program - Korean Ministry of Science, ICT and Future Planning [2013K000275]
- Korea Health Promotion Institute [HI10C1740030014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Science, ICT & Future Planning, Republic of Korea [IBS-R021-D1-2014-A00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2011-50331, 2011-0013568] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Programmable nucleases - including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system - enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site-specific DNA cleavage in the genome, the repair (through endogenous mechanisms) of which allows high-precision genome editing. However, these nucleases differ in several respects, including their composition, targetable sites, specificities and mutation signatures, among other characteristics. Knowledge of nuclease-specific features, as well as of their pros and cons, is essential for researchers to choose the most appropriate tool for a range of applications.
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