Journal
NATURE REVIEWS GENETICS
Volume 12, Issue 2, Pages 87-98Publisher
NATURE RESEARCH
DOI: 10.1038/nrg2934
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Funding
- US National Human Genome Research Institute [R01 HG005230, R44 HG005279]
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In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.
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