Journal
NATURE REVIEWS GENETICS
Volume 10, Issue 1, Pages 57-63Publisher
NATURE RESEARCH
DOI: 10.1038/nrg2484
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Funding
- NATIONAL CANCER INSTITUTE [R01CA077808] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [P50HG002357] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA077808-12, R01 CA077808] Funding Source: Medline
- NHGRI NIH HHS [P50 HG002357, P50 HG002357-10] Funding Source: Medline
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RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.
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