Journal
NATURE PROTOCOLS
Volume 9, Issue 1, Pages 182-192Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2014.004
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Funding
- US National Institutes of Health (NIH) [R01CA055360, 5 P30CA016087-32]
- Canadian Institutes of Health Research postdoctoral fellowship
- American Association for Cancer Research postdoctoral fellowship
- NIH NCI NRSA Individual Fellowship [F32CA171877]
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
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Macropinocytosis serves as an internalization pathway for extracellular fluid and its contents. Macropinocytosis is upregulated in oncogene-expressing cells and, recently, we have revealed a functional role for macropinocytosis in fueling cancer cell growth through the internalization of extracellular albumin, which is degraded into a usable source of intracellular amino acids. Assessing macropinocytosis has been challenging in the past because of the lack of reliable assays capable of quantitatively measuring this uptake mechanism. Here we describe a protocol for visualizing and quantifying the extent of macropinocytosis in cells both in culture and growing in vivo as tumor xenografts. By using this approach, the 'macropinocytic index' of a particular cell line or subcutaneous tumor can be ascertained within 1-2 d. The protocol can be carried out with multiple samples in parallel and can be easily adapted for a variety of cell types and xenograft or allograft mouse models.
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