Journal
NATURE PROTOCOLS
Volume 9, Issue 2, Pages 457-463Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2014.024
Keywords
-
Categories
Funding
- Institut National de la Recherche Agronomique (INRA), France
- Ministry of Science and Higher Education, Poland
- National Science Centre, Poland [2011/02/A/NZ3/00079]
- Agence Nationale de la Recherche [ANR-10-BLAN-1516 'Mechastem']
Ask authors/readers for more resources
Cell biology heavily relies on the behavior of fibrillar structures, such as the cytoskeleton, yet the analysis of their behavior in tissues often remains qualitative. Image analysis tools have been developed to quantify this behavior, but they often involve an image pre-processing stage that may bias the output and/or they require specific software. Here we describe FibrilTool, an ImageJ plug-in based on the concept of nematic tensor, which can provide a quantitative description of the anisotropy of fiber arrays and their average orientation in cells, directly from raw images obtained by any form of microscopy. FibrilTool has been validated on microtubules, actin and cellulose microfibrils, but it may also help analyze other fibrillar structures, such as collagen, or the texture of various materials. The tool is ImageJ-based, and it is therefore freely accessible to the scientific community and does not require specific computational setup. The tool provides the average orientation and anisotropy of fiber arrays in a given region of interest (ROI) in a few seconds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available