Journal
NATURE PROTOCOLS
Volume 8, Issue 11, Pages 2098-2104Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2013.138
Keywords
-
Categories
Funding
- Yokohama City University
- grant for Establishment of Research Center for Clinical Proteomics of Post-translational Modifications
- [20116005]
- [24390371]
- [24770216]
- Grants-in-Aid for Scientific Research [24770216] Funding Source: KAKEN
Ask authors/readers for more resources
The in vitro propagation of mouse spermatogonial stem cells (SSCs) became possible in 2003; these cultured SSCs were named germ-line stem (GS) cells. To date, however, it has not been possible to induce spermatogenesis from GS cells in vitro. Recently, we succeeded in producing functional sperm from primitive spermatogonia in explanted neonatal mouse testis tissues. Here we describe a protocol that can support spermatogenesis from GS cells up to sperm formation in vitro using an organ culture method. GS cells transplanted in the extracted testis form colonies in the tissue fragments and differentiate into sperm under the described in vitro organ culture conditions. It takes about 6 weeks to obtain sperm from GS cells. The sperm are viable, resulting in healthy offspring through micro-insemination. Thus, this protocol should be a valuable tool for the study of mammalian spermatogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available