Journal
SMALL
Volume 12, Issue 7, Pages 902-910Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201502220
Keywords
acoustic streaming; acoustically actuated bubbles; cell deformability; optothermal effects; microfluidics
Categories
Funding
- National Institutes of Health [1 R01 GM112048-01A1, 1R33EB019785-01]
- National Science Foundation [CBET-1438126, IDBR-1455658]
- Penn State Center for Nanoscale Science (MRSEC) [DMR-1420620]
- NSF
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1438126] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1455658] Funding Source: National Science Foundation
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An acoustically actuated, bubble-based technique is developed to investigate the deformability of cells suspended in microfluidic devices. A microsized bubble is generated by an optothermal effect near the targeted cells, which are suspended in a microfluidic chamber. Subsequently, acoustic actuation is employed to create localized acoustic streaming. In turn, the streaming flow results in hydrodynamic forces that deform the cells in situ. The deformability of the cells is indicative of their mechanical properties. The method in this study measures mechanical biomarkers from multiple cells in a single experiment, and it can be conveniently integrated with other bioanalysis and drug-screening platforms. Using this technique, the mean deformability of tens of HeLa, HEK, and HUVEC cells is measured to distinguish their mechanical properties. HeLa cells are deformed upon treatment with Cytochalasin. The technique also reveals the deformability of each subpopulation in a mixed, heterogeneous cell sample by the use of both fluorescent markers and mechanical biomarkers. The technique in this study, apart from being relevant to cell biology, will also enable biophysical cellular diagnosis.
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