Journal
NATURE PROTOCOLS
Volume 7, Issue 11, Pages 1983-1995Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.119
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Funding
- Genentech
- Hertie foundation
- Theodor Korner Fonds
- Austrian Science Fund (FWF) [P23102] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [P 23102] Funding Source: researchfish
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The examination of tissue histology by light microscopy is a fundamental tool for investigating the structure and function of organs under normal and disease states. Many current techniques for tissue sectioning, imaging and analysis are time-consuming, and they present major limitations for 3D tissue reconstruction. The introduction of methods to achieve the optical clearing and subsequent light-sheet laser scanning of entire transparent organs without sectioning represents a major advance in the field. We recently developed a highly reproducible and versatile clearing procedure called 3D imaging of solvent-cleared organs, or 3DISCO, which is applicable to diverse tissues including brain, spinal cord, immune organs and tumors. Here we describe a detailed protocol for performing 3DISCO and present its application to various microscopy techniques, including example results from various mouse tissues. The tissue clearing takes as little as 3 h, and imaging can be completed in similar to 45 min. 3DISCO is a powerful technique that offers 3D histological views of tissues in a fraction of the time and labor required to complete standard histology studies.
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