Journal
NATURE PROTOCOLS
Volume 7, Issue 5, Pages 829-838Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.021
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Funding
- Swiss National Science Foundation [PBBEP3_129803]
- German Research Foundation
- Howard Hughes Medical Institute
- US National Institutes of Health (NIH) [DP2OD004437, RC1AG036142, R01AI085575]
- Burroughs Wellcome Foundation
- Swiss National Science Foundation (SNF) [PBBEP3_129803] Funding Source: Swiss National Science Foundation (SNF)
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Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles platform as a powerful tool for comparatively investigating the regulation of developmental processes in single cells. This approach overcomes the limitations involving heterogeneous cell populations and sample amounts, and may shed light on differential regulation of gene expression in normal versus disease-related contexts. Furthermore, high-throughput single-cell qRT-PCR provides a standardized, comparative assay for in-depth analysis of the mechanisms underlying human pluripotent stem cell self-renewal and differentiation.
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