Journal
NATURE PROTOCOLS
Volume 7, Issue 9, Pages 1755-1764Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.097
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Funding
- Canadian Institutes of Health Research Fellowship
- Kaleidoscope of Hope Young Investigator Research Grant
- US National Institutes of Health [P50 CA105009]
- Ovarian Cancer Research Fund
- Robert and Debra First Fund
- Randi and Joel Cutler Ovarian Cancer Research Fund
- Mary Kay Foundation
- Sandy Rollman Ovarian Cancer Foundation
- Susan Smith Center for Women's Cancers at the Dana-Farber Cancer Institute
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Primary human fallopian tube secretory epithelial cell (FTSEC) cultures are useful for studying normal fallopian tube epithelial biology, as well as for developing models of fallopian tube disease, such as cancer. Because of the limited ability of primary human FTSECs to proliferate in vitro, it is necessary to immortalize them in order to establish a cell line that is suitable for long-term culture and large-scale in vitro experimentation. This protocol describes the isolation of FTSECs from human fallopian tube tissue, conditions for primary FTSEC culture and techniques for establishing immortal FTSEC lines. The entire process, from primary cell isolation to establishment of an immortal cell line, may take up to 2 months. Once established, immortal FTSECs can typically be maintained for at least 30 passages.
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