Journal
NATURE PROTOCOLS
Volume 7, Issue 6, Pages 1207-1218Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.052
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Funding
- Engineering and Physical Sciences Research Council
- Swiss National Science Foundation
- Government of Malaysia
- Medical Research Council Institute of Genetics and Molecular Medicine
- Royal Society
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We have developed miniaturized heterogeneous Pd-0-catalysts (Pd-0-microspheres) with the ability to enter cells, stay harmlessly within the cytosol and mediate efficient bioorthogonal organometallic chemistries (e.g., allylcarbamate cleavage and Suzuki-Miyaura cross-coupling). This approach is a major addition to the toolbox available for performing chemical reactions within cells. Here we describe a full protocol for the synthesis of the Pd-0-microspheres from readily available starting materials (by the synthesis of size-controlled amino-functionalized polystyrene microspheres), as well as for their characterization (electron microscopy and palladium quantitation) and functional validation ('in solution' and 'in cytoplasm' conversions). From the beginning of the synthesis to functional evaluation of the catalytic device requires 5 d of work.
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