Journal
NATURE PROTOCOLS
Volume 5, Issue 4, Pages 725-738Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2010.5
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Funding
- Alfred P. Sloan Foundation
- National Science Foundation (Career) [0746198]
- National Institute of General Medical Sciences [GM083107, GM084222]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM083107, R01GM084222] Funding Source: NIH RePORTER
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function prediction based on the sequence-to-structure-to-function paradigm. Starting from an amino acid sequence, I-TASSER first generates three-dimensional (3D) atomic models from multiple threading alignments and iterative structural assembly simulations. The function of the protein is then inferred by structurally matching the 3D models with other known proteins. The output from a typical server run contains full-length secondary and tertiary structure predictions, and functional annotations on ligand-binding sites, Enzyme Commission numbers and Gene Ontology terms. An estimate of accuracy of the predictions is provided based on the confidence score of the modeling. This protocol provides new insights and guidelines for designing of online server systems for the state-of-the-art protein structure and function predictions. The server is available at http://zhanglab.ccmb.med.umich.edu/I-TASSER.
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