Journal
NATURE PROTOCOLS
Volume 4, Issue 9, Pages 1245-1251Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2009.121
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Funding
- United States Public Health Service [R37 CA090426]
- NATIONAL CANCER INSTITUTE [R37CA090426] Funding Source: NIH RePORTER
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Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH-P450 reductase in biological preparations. A detailed protocol is presented for the measurement of P450 by its spectral properties, along with a protocol for measuring NADPH-P450 reductase by its NADPH-cytochrome c reduction activity. Each assay can be completed in 5-10 min. Detailed explanations for the rationale of particular sequences in the protocols are provided, along with potential confounding problems.
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