Journal
NATURE PROTOCOLS
Volume 4, Issue 1, Pages 78-85Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2008.199
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Funding
- DFG
- Australian Research Council (ARC)
- University of Sydney
- Medical Foundation Fellow
- National Health and Medical Research Council (NHMRC)
- Ministry for Science and Medical Research (BioFirst Program)
- Nerve Research Foundation
- Medical Foundation (University of Sydney)
- Judith Jane Mason and Harold Stannett Williams Memorial Foundation
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Primary cultures of rat and murine hippocampal neurons are widely used to reveal cellular mechanisms in neurobiology. Their use is limited, as culturing at low density is often not possible or is dependent on sophisticated methods. Here we present a novel method for culturing embryonic (E16.5) murine hippocampal neurons, using a spatially separated ring of cortical neurons for neurotrophic support. This method allows long-term cultures at a very low cell density, and therefore, the study of single embryo preparations and isolated neurons. This method has been adopted for neurons from the substantia nigra ( E16.5), with support from a ring of striatal neurons.
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