Journal
NATURE PROTOCOLS
Volume 4, Issue 1, Pages 71-77Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2008.217
Keywords
-
Categories
Funding
- NIH [1 R01 AI066054-01A]
- NSF
- NRI USDA CSREE
- BSF
- BARD
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI066054] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Transient gene expression is an indispensable tool for studying functions of gene products. In the case of plants, transient introduction of genes by Agrobacterium infiltration is a method of choice for many species. However, this technique does not work efficiently in Arabidopsis leaf tissue, the most widely used model system for basic plant biology research. Here we present an optimized protocol for biolistic delivery of plasmid DNA into the epidermis of Arabidopsis leaves, which can be easily performed using the Bio-Rad Helios gene gun system. This protocol yields efficient and reproducible transient expression of diverse genes and is exemplified here for use in a functional assay of a transcription repressor and for the subcellular localization and cell-to-cell movement of plant viral movement protein. This protocol is suitable for studies of biological function and subcellular localization of the gene product of interest directly in planta by utilizing different types of activity-based assays. Using this procedure, the data are obtained after 2-4 d of work.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available