Journal
NATURE PROTOCOLS
Volume 4, Issue 10, Pages 1440-1453Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2009.156
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Funding
- BBSRC [BB/D014808/1]
- NIBIB [1 P20 EB007076-01]
- EU [201842-ENCITE]
- Charles Wolfson Charitable Trust Foundation
- RCUK
- Biotechnology and Biological Sciences Research Council [BB/D014808/1] Funding Source: researchfish
- BBSRC [BB/D014808/1] Funding Source: UKRI
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Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.
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